Effects of CD86 costimulation on the modulation of Th2 bias at maternal-fetal interface and the relationship with outcomes of gestation / 中国免疫学杂志

ABSTRACT

Objective:To explore the role of CD86 costimulation in inducing Th2 bias at maternal-fetal interface and the relationship to outcomes of gestation.Methods:Pregnant DBA/2J mated CBA/J mice with a high embryo resorption rate from 20% to 30% and BALB/C mated CBA/J mice with low embryo resorption rates were studied,with rat anti-murine CD86 mAb administered intraperitoneally at the dosage of 100 ?g,at the time of implantation(day 4) and on the following days(6,8,10) of gestation.The competitive semiquantity RT-PCR and ELISA was applied to analysis of the transcription and expression of Th type-1/Th type-2 cytokines at the maternal-fetal interface at day 9 or day 14 of gestation respectively.The embryo resorption rate was counted at day 14 of gestation.Results:In the model of normal pregnancy,blockade of CD86 costimulation had no significant effects on the original Th2 bias at the maternal-fetal interface,and the outcomes of gestation had not changed significantly.While in the model of abortion-prone,blockade of CD86 costimulation successfully induced a Th2 bias at maternal-fetal interface.Therefore,the embryo resorbing rates decreased significantly.Conclusion:Blocking the CD86 costimulation at the early stage of the abortion-prone pregnancy could recover the physiological balance of Th1/Th2 at maternal-fetal interface and induce the maternal-fetal immune tolerance.