Expression of prepro-orexin mRNA in normal sleep and sleep-deprived rats / 中华神经科杂志
Chinese Journal of Neurology
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-540770
Responsible library:
WPRO
ABSTRACT
Objective To study the expression of prepro-orexin mRNA in circadian rhythms and sleep-deprived rats.Methods Sleep was deprived with “rotation cylinder” in rats. RT-PCR was used to determine the change of prepro-orexin mRNA expression in cortex and hypothalamus of circadian rhythms and sleep-deprived rats.Results There was a significant changes of prepro-orexin mRNA expression with circadian rhythms in hypothalamus of rats. But there was no significant change in cortex. More prepro-orexin mRNA expression was found in hypothalamus than in cortex of rats. The expression of prepro-orexin mRNA in hypothalamus and in cortex of rats was not effected by short times of sleep deprivation. However the expression was raised by a 8-hour sleep deprivation.Conclusions There should be a close relationship between orexin and sleep. The control of sleep may be dependent on regulation of orexin in hypothalamus.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Neurology
Year:
2001
Type:
Article