MUTUAL INTERACTION BETWEEN HEPATITIS B CORE ANTIGEN AND METALLOTHIONEIN / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1983.
Article
in Chinese
| WPRIM
| ID: wpr-553527
ABSTRACT
To investigate the potential role of hepatitis B virus core protein (HBcAg) in the pathogenesis of hepatitis B and seek for an effective therapeatic approach to the treatment of hepatitis B, the improved yeast two hybrid technique was employed to construct HBcAg bait plasmid, and the plasmids were transformed into yeast AH109. Then the yeast AH109 was mated with yeast Y187 containing liver cDNA library plasmid in 2?YPDA medium, diploid yeast was plated on both synthetic dropout nutrient medium (SD/ Trp Leu His Ade) and synthetic dropout nutrient medium (SD/ Trp Leu His Ade) containing X ? gal to be selected and screened two times. Plasmids were extracted from sixteen positive colonies, and they were sequenced. Among them, two colonies contained metallothionein. The interaction between HBcAg and metallothionein was confirmed by in vitro reticulocyte lysate translocation and immunoprecipitation. The results suggested that the pathogenesis of hepatitis B metallothionein might play an important role in the episode process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
1983
Type:
Article
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