Determination of luteolin and isorhamnetin in Duyiwei Capsules by HPLC / 中成药

ABSTRACT

AIM: To establish the method for determination of luteolin and isorhamnetin in Duyiwei Capsules (Lamiophl mis rotata(Benth.)Kudo). METHODS:Yilite-C_(18) column was used.The mobile phase was composed of methanol-water(50∶50),pH was adjusted to 3.0 with H_3PO_4.The flow rate was 0.8 mL?min~(-1) and detection wave-length was set at 360 nm. RESULTS:The linear range of Luteolin was 7.072-35.360 ?g?mL~(-1),(r=(0.999 7)) and the linear range of isorhamnetin was 1.658-8.290 ?g?mL~(-1)(r=0.999 2). The average recovery and RSD of luteolin were 96.3% and 2.7%,respectively. The average recovery and RSD of isorhamnetin were 97.3% and 2.1%,respectively. CONCLUSION:This method is sensitive,accurate and can be used for the quality study of Duyiwei Capsules.