Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor / 重庆医科大学学报

ABSTRACT

Objective: To establish the model of cultured human amniotic epithelial cells(HAECs) on the fibrin clot in order to observe the effect of EGF,bFGF and TGF-?1 on HAEC.Methods:The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microscopy,Giemsa staining and scanning electron microscopy.The effect of growth factor(EGF,bFGF,and TGF-?1) on the apoptosis of HAECs was measured by TUNEL.Results: HAECs generated on the formulated fibrin clot in vitro.HAECs exposed to EGF or bFGF showed a lower rate of apoptosis than the control group(P