Metabolism of Chrysanthemum morifolium extracts in intestinal flora / 中草药

ABSTRACT

Objective To study and compare the metabolism of Chrysanthemum morifolium extracts(CME) in vitro intestinal flora from human,rat,Beagle dog,and rabbit.Methods Intestinal flora of human,rat,Beagle dog,and rabbit and CME were anaerobically cultured at 37 ℃ in vitro. After being extracted by acetic ether,the main metabolites of CME were separated and quantified by HPLC.Results In vitro,CME was easily metabolized by the intestinal flora and the main metabolites in incubation medium were determined in high concentration after 0.5 h.By LC-MS,luteolin and apigenin were identified,respectively;both metabolites were degraded with prolongation of incubation time and the concentrations of luteolin and apigenin were low after 24 h;moreover,CME was quickly metabolized by human,rat,Beagle dog intestinal flora,and gently by rabbit intesinal flora.Conclusion In vitro,CME is easily metabolized by intestinal flora human,rat,Beagle dog,and rabbit and the main metabolites are luteolin and apigenin.