Separation and purification of Naomaitong Granules by AB-8 macroporous absorption resin / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
in Zh
| WPRIM
| ID: wpr-579123
Responsible library:
WPRO
ABSTRACT
AIM:
To study the purification of Naomaitong Granules(Radix et Rhizoma rhei,Rhizoma chuanxiong,Radix Puerariae lobatae.etc) by macroporous absorption resin.METHODS:
Naomaitong Granule was purificated by macroporous absorption resin AB-8.UV spectrothotometry was used to determine the contents of total anthraquinones,total ginsenosides,total alkaloids,and the content of puerarin was determined by HPLC.The technic of purification was optimized according to the content above.RESULTS:
The optimized technological conditions consisted of eoncentranon of original sample 120 mg/mL,the diameter and height was in proportion of 1∶10,the ratio of maximum adsorption to resin volume was 1∶6,water elution was 2B multiple of resin volume,8B multiple of resin volume 50% alcohol was the elution.CONCLUSION:
AB-8 macroporous absorption resin can be used to purify Naomaitong Granules.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article