Your browser doesn't support javascript.
loading
Effects of Scutellarin on MUC5AC Mucin Production Induced by Human Neutrophil Elastase or Interleukin 13 on Airway Epithelial Cells
Journal of Korean Medical Science ; : 778-784, 2011.
Article in English | WPRIM | ID: wpr-58123
ABSTRACT
Scutellarin is a flavonoid extracted from a traditional Chinese herb, Erigeron breviscapus. The present study investigated the effect of scutellarin on MUC5AC mucin production and the possible mechanism. Human bronchial epithelial 16 (HBE16) cells were pretreated with scutellarin for 60 min, and then exposed to human neutrophil elastase (HNE) or interleukin (IL)-13 for 12 hr. RT-PCR and ELISA were performed to measure the amount of MUC5AC mucin production. The results showed that scutellarin inhibited MUC5AC expression both in mRNA and protein level induced by HNE in a concentration-dependent manner. However, scutellarin failed to inhibit MUC5AC mucin production induced by IL-13. To investigate the intracellular mechanisms associated with the effect of scutellarin on MUC5AC mucin production, western blotting was carried out to examine the phosphorylation of protein kinase C (PKC), signal transducer and activator of transcription 6 (STAT6) and extracellular signal-regulated kinase 1/2 (ERK1/2). The phosphorylation of PKC and ERK1/2 was attenuated after treatment with scutellarin, whereas STAT6 was not significantly affected. Therefore, it is suggested that scutellarin down-regulates MUC5AC mucin production on HBE16 cells via ERK-dependent and PKC-dependent pathways.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Protein Kinase C / Signal Transduction / Down-Regulation / Cells, Cultured / Interleukin-13 / Leukocyte Elastase / Mitogen-Activated Protein Kinase 1 / Respiratory Mucosa / Apigenin Limits: Humans Language: English Journal: Journal of Korean Medical Science Year: 2011 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Phosphorylation / Protein Kinase C / Signal Transduction / Down-Regulation / Cells, Cultured / Interleukin-13 / Leukocyte Elastase / Mitogen-Activated Protein Kinase 1 / Respiratory Mucosa / Apigenin Limits: Humans Language: English Journal: Journal of Korean Medical Science Year: 2011 Type: Article