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Simultaneous determination of four constituents in Compound Kendir Leaves Tablets Ⅰ by HPLC-DAD / 中成药
Chinese Traditional Patent Medicine ; (12): 1625-1628, 2017.
Article in Chinese | WPRIM | ID: wpr-609439
ABSTRACT
AIM To establish an HPLC-DAD method for the simultaneous content determination of four constituents in Compound Kendir Leaves Tablets Ⅰ (Apocyni veneti Folium,Chrysanthemi indici Flos,Stephaniae tetrandrae Radix,etc.).METHODS The analysis of 50% methanol extract of this drug was performed on a 35 ℃ thermostatic Shimadzu VP-ODS column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of methanol-0.5% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 260 nm and 325 nm.RESULTS Chlorogenic acid,hydrochlorothiazide,buddleodide and promethazine hydrochloride showed good linear relationships within the ranges of 24.91-498.2 ng (r =0.999 9),286.33-5 726.7 ng (r =0.999 9),10.04-200.9 ng (r =0.999 9) and 154.80-3 096.1 ng (r =0.999 9),whose average recoveries were 98.3% (RSD =1.3%),99.1% (RSD =0.6%),98.5% (RSD =1.0%) and 99.3% (RSD =1.2%),respectively.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Compound Kendir Leaves Tablets Ⅰ.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article