Impact of Wildtype p53 Induced Phosphatase 1 Gene Lacking on Heart Function in Experimental Mice / 中国循环杂志
Chinese Circulation Journal
; (12): 792-796, 2017.
Article
in Zh
| WPRIM
| ID: wpr-614142
Responsible library:
WPRO
ABSTRACT
Objective: To explore the impact of knocking out wildtype p53 phosphatase 1 gene on heart function with the changes of cardiac tissue mRNA and protein expressions in experimental mice. Methods: Our research included in 2 groups: Wildtype (WT) mice group and Wip1 knockout (Wip1-KO) mice group. n=10 in each group. The heart function, ratio of heart weight to body weight (HW/BW) were examined and compared between 2 groups; cardiac tissue morphology was observed by HE staining; mRNA expressions of ANP, BNP, MCP-1 andα-SMA were determined by RT-PCR and protein expressions of Bcl-2, Bax and c-caspase3 were measured by Western blot analysis. Results: Compared with WT mice group, Wip1-KO mice group showed decreased Wip1 mRNA expression,P0.05; apoptosis related protein expressions of Bax/Bcl-2 and c-caspase3 were similar between 2 groups,P>0.05. Conclusion: Wip1 gene knockout may impair the heart function in experimental mice, while the relevant mechanism should be further investigated.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Circulation Journal
Year:
2017
Type:
Article