Nutlin-3 promotes pyroptosis in SMMC-7721 cells / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1014-1017, 2017.
Article
in Zh
| WPRIM
| ID: wpr-616460
Responsible library:
WPRO
ABSTRACT
Objective:
To evaluate the effect of Nutlin-3,a MDM2 antagonist,on the pyroptosis in SMMC-7721 cells.Methods:
The expression of actived caspase-1(p20) and IL-1β was detected using Western blot analysis.Pyroptosis was investigated by a standard lactate dehydrogenase release assay(LDH).IL-1β content in the cell culture supernatant was quantified by ELISA.Results:
Nutlin-3 up-regulated the expression level of actived caspase-1 and IL-1β in SMMC-7721 cells.Meanwhile,Nutlin-3 increased significantly the content of LDH and IL-1β in the cell culture supernatant(P<0.05).Conclusion:
Nutlin-3 activated pro-caspase-1,promoted pyroptosis and IL-1β release in SMMC-7721 cells.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
2017
Type:
Article