Effect of hydrogen on endoplasmic reticulum stress during liver cold ischemia-reperfusion in rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of hydrogen on the endoplasmic reticulum stress during liver cold ischemia-reperfusion (I/R) in rats.Methods Twenty-four SPF healthy adult male Sprague-Dawley rats,weighing 200-250 g,were divided into 3 groups (n=8 each) using a random number tablesham operation group (group S),liver cold I/R group (group I/R) and hydrogen-saturated lactated Ringer's solution group (group HL).After occlusion of the liver blood flow,livers were perfused through the portal vein with 4 ℃ hydrogen-saturated lactated Ringer's solution 6-8 ml/min for 30 min.The liver blood flow was restored after the end of perfusion.At 6 h of reperfusion in I/R and HL groups or at 6 h after peritoneum closure in group S,blood samples from the inferior vena cava were collected for determination of serum alanine transaminase (ALT) and aspartate transaminase (AST) concentrations.After blood sampling,liver tissues were obtained for examination of pathological changes and for determination of cell apoptosis (using TUNEL),malondialdehyde (MDA) content (by thiobarbituric acid method),superoxide dismutase (SOD) activity (using xanthine oxidase method),expression of endoplasmic reticulum protein 46 (ERP46),immunoglobulin heavy chain binding protein (BiP) and caspase-12 (by immunohistochemistry),and expression of ERP46,BiP and caspase-12 mRNA (using quantitative real-time polymerase chain reaction).The pathological changes were scored.Apoptosis rate was calculated.Results Compared with group S,the serum ALT and AST concentrations,pathological scores,apoptosis rate and MDA content were significantly increased,the SOD activity was decreased,and the expression of ERP46,BiP and caspase-12 protein and mRNA was up-regulated in I/R and HL groups (P＜0.05).Compared with group I/R,the serum ALT and AST concentrations,pathological scores,apoptosis rate and MDA content were significantly decreased,the SOD activity was increased,and the expression of ERP46,BiP and caspase-12 protein and mRNA was down-regulated in group HL (P＜0.05).Conclusion The mechanism by which hydrogen reduces cell apoptosis may be related to inhibition of endoplasmic reticulum stress during liver cold I/R in rats.