Performance of LIFECODES HLA-DQB1 Typing Kit Using Luminex Platform in Koreans
Annals of Laboratory Medicine
;
: 123-127, 2015.
Article
in English
| WPRIM
| ID: wpr-64360
ABSTRACT
Intermediate-resolution HLA-DQ typing has gained importance in organ transplantation recently. We evaluated the performance of the LIFECODES HLA-DQB1 typing kit (Immucor, USA) using sequence-specific oligonucleotide (SSO) probe and Luminex platform (Luminex Corp., USA) on 100 samples tested by sequence-based typing (SBT) using the AlleleSEQR HLA-DQB1 kit (Abbott Molecular, USA) in Korean individuals. No sample showed ambiguity in the assignment of 4-digit HLA-DQB1 allele with the LIFECODES HLA-DQB1 SSO typing kit, and the results were fully concordant with those of high-resolution typing of AlleleSEQR HLA-DQB1 SBT up to 4-digit level. Three samples required adjustment of false reactions (3/100, 3.0%) two samples with DQB1*0303/*0601 showed false-positive result in probe 253, and 1 sample with DQB1*0402/*0502 showed false-negative result in probe 217. We tested an additional sample with DQB1*0303/*0601, which showed same false-positivity in probe 253 and 2 samples with DQB1*0402/*0502, which showed no false reaction. The false reactions did not result in ambiguity or change in the HLA allele assignment. We could assign HLA-DQB1 alleles to 4 digit-level without ambiguity, with 100% concordance with the SBT results. Thus, LIFECODES HLA-DQB1 SSO typing kit showed good performance for intermediate-resolution HLA-DQB1 typing in clinical laboratory for organ transplantation in Koreans.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Histocompatibility Testing
/
Polymerase Chain Reaction
/
DNA Primers
/
Alleles
/
Republic of Korea
/
HLA-DQ beta-Chains
/
Gene Frequency
/
Genotype
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2015
Type:
Article
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