Expression of SIX1 in cervical cancer and relationship with cervical cancer cell proliferation / 临床与实验病理学杂志

ABSTRACT

Purpose To investigate the expression of SIX1 in cervical cancer and its biological function.Methods The expression of SIX1 and Ki-67 in 35 cervical cancer tissues,42 cervical intraepithelial neoplasia (CIN) tissues and 15 normal cervical tissues was detected by immunohistochemical of EliVision two-step staining.A plasmid vector expressing SIX1 siRNA was constructed and then stably transfected into Hela cells.The expression of SIX1 mRNA and protein was examined by RT-PCR and Western blot method respectively.The proliferation was exanined by MTT.Results The positive rate of SIX1 in cervical cancer was significantly higher than that of CIN and normal cervical tissues and was positively correlation with the proliferation index of Ki-67.After RNAi treatment,the mRNA and protein levels of SIX1 was down-regulated in Hela cells and the proliferation of Hela cells was inhibited.Conclusion SIX1 may play an important role in the occunence and development process of cervical cancer by promoting cervical cancer cell proliferation.