Effect of bone-marrow mesenchymal stem cell transplantation on behavioral changes in mice after ischemic brain injury / 中华行为医学与脑科学杂志

ABSTRACT

Objective To study the effect of bone-marrow mesenchymal stem cell (BMSC) via artery transplantation on behavior changes after ischemic brain injury in mice.Methods 60 mice (C57BL/6) were divided randomly into sham group,brain ischemia group (MCAO group) and stem cell therapy group (BMSC group).The latter two groups were injected respectively with 200 μl PBS or BMSC suspension into common carotid arteries namely when removal suture after middle cerebral artery occlusion model,while sham group was only isolated carotid artery.Infarct size of brain tissue was measured by TTC staining.Focal deficit score,Morris water maze test,the rotating beam test and Rotarod test were resepectively made to evaluate the animal behavior after injury.Results Adequate amounts of BMSCs were harvested by adherence screening method and subculture.Ischemic area of BMSC group ((34.98±12.49) ％) was significantly smaller than that of MCAO group ((42.36±9.41)％) at 2nd day after injury (P＜0.05).Compared with MCAO group,focal deficit score of BMSC group reduced significantly at 3rd day after injury,and got to the most significant differences at 5th day after injury (P＜0.01);escape latency of BMSC group was significantly shortened at 7th day and 14th day after injury in Morris water maze test (P＜ 0.05),meanwhile time percentage,distance percentage in the target quadrant and the times corssing the platform were increased gradually after injury,and reached significant differences at 14th day after injury(P＜0.05);exercise time in Rotarod runner increased at every time point after injury,and reached most significant differences at 14th day after injury(P＜0.01);walking speed in the rotating beam test increased most significantly at 14th day after injury,meanwhile walking distance at 5th and 10th day after injury(P＜0.01).Conclusion BMSC transplantation via carotid artery can significantly improve neural function,learning,balance and motor function after brain injury,which will be a new way of TBI therapy.