Isolation and purification of basement membrane zone mono-specific autoantibodies by spot affinity purified method / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1985.
Article
in Zh
| WPRIM
| ID: wpr-674789
Responsible library:
WPRO
ABSTRACT
Objective:To investigate the value of spot affinity purified method in the isolation and purification of mono specific basement membrane zone autoantibodies(BMZ Ab).Methods:Utilizing human dermoepidermal antigen transmitted on NC membrane,We purified 3 cases with mono specific BMZ Ab in sera of SABD patients which was oppositive by immunoblotting.Results:The BMZ Ab purified by this method could specifically combine its target antigen.Conclusion:Spot affinity purified method could be widely used in the isolation and purification of autoantibodies produced by mixed micro antigen.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article