Effect of blocking Wnt-?-catenin signaling pathway on activation of hepatic stellate cells / 中华传染病杂志
Chinese Journal of Infectious Diseases
; (12)2007.
Article
in Zh
| WPRIM
| ID: wpr-679881
Responsible library:
WPRO
ABSTRACT
Objective To investigate whether functional Wnt-?-catenin signaling is present in activated hepatic stellate cells (HSC),and the effect of blocking this signaling on activation of HSC. Methods?-catenin expression in HSC was examined by immunocytochemistry.Wnt signalings in HSC-T6 were assessed using a T cell factor (TCF)-dependent luciferase reporter gene (pTOP- FLASH) assay.Wnt signalings in HSC-T6 were blocked by transfecting with a dominant negative TCF (dnTCF) expression plasmid,then the expression of alpha smooth muscle actin (?-SMA) and collagen typeⅠwere examined by Western blot.Results?-catenin staining was positive in the nuclei of HSC-T6.Luciferase activity in the cells transfected with pTOPFLASH was significantly higher than that in the cells transfected with pFOPFLASH (P
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Infectious Diseases
Year:
2007
Type:
Article