The Identification of Antagonistic Bacteria B-04 against Botrytis cinerea and Improving Biocontrol Effect through Genetic Modification with ?-1,3-glucanase Gene / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-685403
Responsible library:
WPRO
ABSTRACT
Bacillus cereus B-04 antagonist to Botrytis cinerea were isolated from samples of tomato soil infected by Botrytis cinerea in Zibo, which are identified through a series of morphological and biochemical characteristics and the sequence of 16SrDNA. Aiming at enhancing the inhibitory effect of this strain, a 4.1kb DNA fragment containing ?-1,3-glucanases gene from pUC1940 was inserted into vector pBE2 and pHY300PLK to construct recombination plasmids, PBE2-glu and pHY300PLK-glu, which were transferred into Bacillus cereus B-04, resulting in a new strain named B-04-glu. Restriction enzyme digestion and ?-1,3-glucanases plate culture confirmed that B-04-glu contained a functional ?-1,3-glucanases gene. Compared to the wild strain B-04, B-04-glu had an increased inhibitory effect against Botrytis cinerea on tomato.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
Zh
Journal:
China Biotechnology
Year:
2006
Type:
Article