Role of BET Bromodomain in Hematopoietic Differentiation from hESCs / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1186-1193, 2018.
Article
in Zh
| WPRIM
| ID: wpr-689508
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of bromodomain and extra terminal (BET) bromodomain in hematopoietic differentiation from human enbryonic stem cells (hESC).</p><p><b>METHODS</b>The effect of BET hematopoietic inhibitor I-BET151 on hematopoietic differentiation from hESC was detected by using a monolayer hematopoietic defferentiation model, immunofluorescence, flow cytometry and real-time PCR; moreover the role of I-BET151 in process of hematopoietic differentiation was explored by adding I-BET151 in different differentiation stages.</p><p><b>RESULTS</b>The analysis results of immunofluorescence, flow cytometry and real-time PCR showed that I-BET 151 significantly inhibited the generation of CD43 positive hematopoietic stem and progenitor cells (HSPCs). It was found that the addition of I-BET 151 in different stages, including APLNR lateral plate mesoderm production, CD34CD31 hemogenic endothelium (HEP) generation and endothelial-to-hematopoietic transition, significantly suppressed the generation of CD43 positive hematopoietic progenitor cells.</p><p><b>CONCLUSION</b>I-BET 151 inhibites hematopoietic differentiation from hESCs at several stages, suggesting that the BET bromodomain plays important roles in multiple stages of hematopoietic differentiation from hESCs.</p>
Full text:
1
Index:
WPRIM
Main subject:
Hematopoietic Stem Cells
/
Cell Differentiation
/
Hemangioblasts
/
Flow Cytometry
/
Human Embryonic Stem Cells
/
Apelin Receptors
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2018
Type:
Article