Expression and Significance of TIPE2 in Ulcerative Colitis / 胃肠病学

ABSTRACT

Background:TIPE2 is a newly identified negative regulator of innate and adaptive immunity that maintains immune homeostasis and immune tolerance. It has been demonstrated that TIPE2 is expressed in a wide variety of tissues and organs in humans. Aims:To investigate the expression of TIPE2 in peripheral blood and colonic mucosa of patients with ulcerative colitis(UC)and non-UC subjects,and to explore the role of TIPE2 in the initiation and development of UC. Methods:Forty-two peripheral blood samples and 30 colonic mucosa samples from patients with active UC were collected during Jan. 2015 to Aug. 2016 at the Second Affiliated Hospital of Xian Jiaotong University. Peripheral blood and colonic mucosa samples from non-UC subjects were served as controls. Real-time PCR and immunohistochemical staining were used to detect the mRNA and protein expressions of TIPE2 in peripheral blood mononuclear cells and colonic mucosal tissues, respectively. Results:Expression level of TIPE2 mRNA in peripheral blood of UC patients had a trend to increase but no significant difference was found between UC patients and the controls(P >0.05). When classified by Truelove-Witts severity index,there was no significant difference among patients with severe,moderate and mild UC(P >0. 05). Expression level of TIPE2 protein was significantly higher in colonic mucosa of UC patients than that of the controls(P<0.05);the expression level increased with increase of histological grade of UC,but the difference was not statistically significant(P > 0.05). Conclusions:Increased colonic expression of TIPE2 might contribute to the initiation and development of UC.