Effects and mechanism of resveratrol on apoptosis of vascular smooth muscle cell in diabetic rats / 中国免疫学杂志

ABSTRACT

Objective:To explore effects and mechanism of resveratrol on apoptosis of vascular smooth muscle cell in diabetic rats. Methods: The mRNA level of monocyte chemoattractant protein 1 (MCP1),macrophage migration inhibitory factor (MIF) and in-terleukin 18 (IL-18) was detected by quantitative real-time reverse transcription PCR (qRT-PCR). Myocardial fibrosis was analyzed by hematoxylin-eosin (HE) staining and Masson staining. Apoptosis was tested by flow cytometry. The expression of Cleaved caspase-3, Cleaved caspase-9,B cell lymphoma 2 (Bcl-2),Bcl-2-associated X protein (Bax),PI3K,p-PI3K,AKT and p-AKT was measured by Western blot. Results: The mRNA levels of MCP1,MIF and IL-18 in STZ-induced diabetic were reduced by resveratrol (P＜0. 05). The aggravation of myocardial fibrosis in STZ-induced diabetic rats was ameliorated by resveratrol. Apoptosis of vascular smooth muscle cell in STZ-induced diabetic rat group was higher than control group (P＜0. 05 ) . Compared with STZ-induced diabetic rat group, apoptosis of vascular smooth muscle cell in STZ+ resveratrol group was decreased (P＜0. 05). What′s more,the expression of Cleaved caspase-3,Cleaved caspase-9 and Bax in STZ-induced diabetic rats were inhibited by resveratrol(P＜0. 05). And the expression of Bcl-2 in STZ-induced diabetic rats was elevated by resveratrol(P＜0. 05). Compared with control group,the rate of p-PI3K/PI3K and p-AKT/AKT in STZ-induced diabetic rat group was decreased (P＜0. 05). The rate of p-PI3K/PI3K and p-AKT/AKT in STZ+ resveratrol group was higher than STZ-induced diabetic rat group ( P＜0. 05). Conclusion: Resveratrol represses apoptosis of vascular smooth muscle cell in STZ-induced diabetic rats through activating of PI3K/AKT signal pathway.