Establishment and application of a TaqMan real-time fluorescence quantitative PCR for detection of tree shrew adenovirus(TAV) / 中国比较医学杂志
Chinese Journal of Comparative Medicine
; (6): 72-77, 2018.
Article
in Zh
| WPRIM
| ID: wpr-703300
Responsible library:
WPRO
ABSTRACT
Objective To establish a quick and accurate method for detection of tree shrew adenovirus(TAV) using TaqMan real-time fluorescence quantitative PCR. Methods Based on the published TAV genome sequence, a 3' conserved sequence was used to design specific probe primers. A standard curve was prepared using a recombinant plasmid containing the target gene fragment. A real-time fluorescence quantitative PCR method was established for detecting TAV based on TaqMan probe. Results The detection method was specific and was not cross-reactive with other common pathogens. The detection limit of the method was 3.7 copies/μL,showing a high sensitivity. The correlation coefficient was 0.998, and the efficiency was 95.7%. The amplification result showed a fine linear relationship,and the repeatability test effect was good. Conclusions The TAV real-time quantitative PCR detection method based on TaqMan probe has been successfully established. It has high sensitivity and reproducibility and can be applied to early detection of TAV infection.
Full text:
1
Index:
WPRIM
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
Zh
Journal:
Chinese Journal of Comparative Medicine
Year:
2018
Type:
Article