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Peptide Nucleic Acid Probe-Based Analysis as a New Detection Method for Clarithromycin Resistance in Helicobacter pylori
Gut and Liver ; : 641-647, 2018.
Article in English | WPRIM | ID: wpr-718123
ABSTRACT
BACKGROUND/

AIMS:

Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important.

METHODS:

We evaluated 70 H. pylori isolates from Korean patients. Dual-labeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing.

RESULTS:

Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (κ-value=0.990; standard error, 0.010).

CONCLUSIONS:

H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA / Helicobacter pylori / Helicobacter / Point Mutation / Clarithromycin / Peptide Nucleic Acids / Fluorescence / Freezing / Methods Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Gut and Liver Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA / Helicobacter pylori / Helicobacter / Point Mutation / Clarithromycin / Peptide Nucleic Acids / Fluorescence / Freezing / Methods Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Gut and Liver Year: 2018 Type: Article