Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status
Annals of Laboratory Medicine
; : 34-38, 2017.
Article
in En
| WPRIM
| ID: wpr-72420
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
The associations of vitamin D deficiency with various clinical conditions highlighted the importance of vitamin D testing. Currently, clinicians measure only the total 25-hydroxyvitamin D [25(OH)D] concentration, regardless of its bioavailability. We aimed to determine the effect of vitamin D-binding protein (VDBP) on 25(OH)D bioavailability.METHODS:
Serum samples were collected from 60 healthy controls, 50 pregnant women, and 50 patients in intensive care units (ICUs). Total 25(OH)D was quantified by liquid chromatography with tandem mass spectrometry, and VDBP levels were determined by using an ELISA kit (R&D Systems, USA). The bioavailable 25(OH)D levels were calculated by using total 25(OH)D, VDBP, and albumin concentrations.RESULTS:
In comparison with healthy controls, the total 25(OH)D concentration was significantly lower in ICU patients (median, 11.65 vs 18.25 ng/mL; P<0.00001), but no significant difference was noted between pregnant women (18.25 ng/mL) and healthy controls. The VDBP level was significantly lower in ICU patients (95.58 vs 167.18 µg/mL, P=0.0002) and higher in pregnant women (225.01 vs 167.18 µg/mL, P=0.008) compared with healthy controls. Nonetheless, the calculated bioavailable 25(OH)D levels of ICU patients and pregnant women were significantly lower than those of healthy controls (1.97 and 1.93 ng/mL vs 2.56 ng/mL; P=0.0073 and 0.0027).CONCLUSIONS:
A single marker of the total 25(OH)D level is not sufficient to accurately evaluate vitamin D status, especially in pregnant women. In cases where VDBP concentrations may be altered, VDBP measurements and bioavailable 25(OH)D calculations may help to determine vitamin D status accurately.Key words
Full text:
1
Index:
WPRIM
Main subject:
Vitamin D
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Vitamin D-Binding Protein
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Serum Albumin
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Enzyme-Linked Immunosorbent Assay
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Chromatography, High Pressure Liquid
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Pregnant Women
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Tandem Mass Spectrometry
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Intensive Care Units
Limits:
Adult
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Aged
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Female
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Humans
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Male
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Pregnancy
Language:
En
Journal:
Annals of Laboratory Medicine
Year:
2017
Type:
Article