Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm
Journal of Veterinary Science
; : 79-86, 2019.
Article
in En
| WPRIM
| ID: wpr-758880
Responsible library:
WPRO
ABSTRACT
The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control (p < 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator (ROMO1) and pro-apoptotic gene (BAX) expressions, together with higher expressions of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic B-cell lymphoma-2 (BCL2), and sperm acrosome associated-3 (SPACA3) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Sperm Motility
/
Spermatozoa
/
Acrosome
/
B-Lymphocytes
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Cryopreservation
/
Reactive Oxygen Species
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2019
Type:
Article