Your browser doesn't support javascript.
loading
Effect of Angelica sinensis polysaccharide on the osteogenic differentiation of bone marrow mesenchymal stem cells of rats with high glucose levels / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 193-199, 2019.
Article in Chinese | WPRIM | ID: wpr-772676
ABSTRACT
OBJECTIVE@#This study aims to evaluate the effect of Angelica sinensis polysaccharide (ASP) on the osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) of rats with high glucose levels.@*METHODS@#Rat BMSCs were isolated and identified by osteogenic and adipogenic differentiation. Then, the BMSCs were divided into three groups as follows normal control group (5.5 mmol·L⁻¹ glucose), high glucose group (25.5 mmol·L⁻¹ glucose), and ASP+high glucose group (25.5 mmol·L⁻¹ glucose +40 mg·L⁻¹ ASP). The proliferation activities of the BMSCs were detected by CCK8. Alizarin red staining, and alkaline phosphatase activity were used in the examination of osteogenic activity. Quantitative real time-polymerase chain reaction was used to detect the expression levels of the osteogenic genes (Runx2, Osx, OCN, Col-Ⅰ) and the key factors of Wnt/β-catenin signal pathway (CyclinD1, β-catenin). In vivo, a type 2 diabetes rat model was established. The rats were divided into three groups, namely, the normal control group (normal rats), diabetes group (diabetic rats), diabetes+ASP group (diabetic rats, ASP feeding). Then, the tibia bone defect was established. The repair of bone defects in each group was observed through histological examination.@*RESULTS@#The proliferation of BMSCs was higher in the high glucose group and ASP+high glucose group than in the normal control group (P0.05). The number of calcium nodules of BMSCs; alkaline phosphatase activity; and the mRNA expression of Runx2, OCN, Osx, Col-Ⅰ, CyclinD1, β-catenin in the high glucose group were lower than those in the normal control and ASP+high glucose groups (P0.05). The bone mass was significantly lower in the bone defect of the diabetes group than in the bone defect of the normal control or diabetes+ASP group (P0.05).@*CONCLUSIONS@#ASP can promote the osteogenic differentiation of rat BMSCs under high glucose culture and induce bone regeneration in rats with type 2 diabetes. These features may be related to the activation of the Wnt/β-catenin signaling pathway.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Pharmacology / Polysaccharides / Bone Marrow Cells / Plant Extracts / Cell Differentiation / Cells, Cultured / Chemistry / Angelica sinensis / Diabetes Mellitus, Experimental Type of study: Prognostic study Limits: Animals Language: Chinese Journal: West China Journal of Stomatology Year: 2019 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Pharmacology / Polysaccharides / Bone Marrow Cells / Plant Extracts / Cell Differentiation / Cells, Cultured / Chemistry / Angelica sinensis / Diabetes Mellitus, Experimental Type of study: Prognostic study Limits: Animals Language: Chinese Journal: West China Journal of Stomatology Year: 2019 Type: Article