Effect of Quercetin on Apoptosis of Platelets and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1612-1616, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-775676
ABSTRACT
OBJECTIVE@#To investigate the effects of quercetin on the apoptosis of platelets and to analyze the intrinsic mechanism.@*METHODS@#Firstly, the effects of quecetin on the apoptosis of platelets was detected by flow cytometry. Secondly, Western blot was used to detect the expression of apoptosis-related proteins in the platelets treated with quercetin for 2 and 4 day.@*RESULTS@#By flow cytometry, it was found that the apoptosis of platelets in the quercetin-treated group (2, 4 and 8 μmol/L) was inhibited, the apoptosis rate of platelets in 2, 4 and 8 μmol/L quercetin group was 3.12%±0.32%, 2.89%±0.15% and 2.31%±0.28%, respectively, which were signigicantly lover than that in control group (P<0.01). With the increase of quecetin concentration, the proportion ratio of platelets significantly decreased in a concentration-dependent manner(r=-0.9985). Similar results were observed on the 4th day. Western blot showed that the treatment with quercetin (2, 4 and 8 μmol/L) promoted the expression of anti-apoptotic protein BCL-2, inhibited the expression of pro-apoptotic protein BAX, resulting in a significant increase in the ratio of BCL-2/BAX (P<0.01), thereby inhibiting the apoptosis of platelets. Similar results were observed on the 4th day.@*CONCLUSION@#Quercetin can inhibit platelet apoptosis by increasing the ratio of apoptosis-related protein BCL-2/BAX in a concentration-dependent manner.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quercetin
/
Blood Platelets
/
Apoptosis
/
Apoptosis Regulatory Proteins
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2019
Type:
Article
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