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Expression Analysis and Epigenetics of MicroRNA-28-5p in Multiple Myeloma / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1540-1547, 2019.
Article in Chinese | WPRIM | ID: wpr-775688
ABSTRACT
OBJECTIVE@#To investigate the expression, mechanism and methylation level of miR-28-5p in multiple myeloma (MM), so as to provide the expirement basis for searching new targeted therapy.@*METHODS@#RT-PCR was used to detect the expression levels of miR-28-5p and potential target CCND1 in CD138 cells of the patients with MM and bone marrow mononuclear cells of patients with iron defficiency anemia(IDA) as control, Methylation-specific PCR(MSP) was used to detect methylation levels of CpG island in LPP/miR-28-5p promoter region and the correlation with other clinical indicators was analyzed. The 5-aza-2'-deoxycytidine (5-Aza-dC,DAC) was used to treat MM cell line U266; after drug treatment,MSP was used to analyze the methylation status of the CpG islands in LPP/miR-28-5p promoter; the qPCR was used to detect the expression levels of miR-28-5p,and the regulatory mechanism of miR-28-5p expression was explored furtherly.@*RESULTS@#The methylation level of CpG island in LPP/miR-28-5p promoter region of MM patients was significantly higher than that of IDA patients. The relative expression level of miR-28-5p in MM patients was significantly lower than that of IDA patients. The relative expression level of miR-28-5p in newly diagnosed MM patients was higher than that in relapsed/progressive patients. The miR-28-5p target CCND1 was expressed at high levels in MM patients with LPP / miR-28-5p methylation, the expression level of miR-28-5p in MM patients correlated with β-MG concentration. 5-aza-dc could significantly inhibit the growth of U266 cell line, arrest the cell cycle in G phase, inhibit the biosynthesis of cellular RNA and protein and promote cell apoptosis. At the same time, up-regulation of miR-28-5p expression was found.@*CONCLUSION@#The expression of miR-28-5p in MM patients is regulated by methylation of CpG islands in the promoter region of the genome.miR-28-5p may act as a tumor suppressor gene, and its low expression may be involved in the occurrence and development of MM, suggesting that miR-28-5p may become a new target for the treatment of MM.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Gene Expression Regulation, Neoplastic / CpG Islands / DNA Methylation / MicroRNAs / Cell Line, Tumor / Epigenesis, Genetic / Genetics / Multiple Myeloma Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Gene Expression Regulation, Neoplastic / CpG Islands / DNA Methylation / MicroRNAs / Cell Line, Tumor / Epigenesis, Genetic / Genetics / Multiple Myeloma Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2019 Type: Article