Quality standards for Kangkeling mixture / 药学实践杂志

ABSTRACT

Objective To establish quality standards for Kangkeling mixture .Methods Ardisiae Japonicae Herba ,Ste-monae Radix were identified by TLC .The content of bergenin was determined by HPLC .Lichrospher C18 column(5 μm , 4 .6 mm × 250 mm)was used with methanol-water (20:80) as mobile phase .The flow rate was 1 .0 ml/min .Column temper-ature was 30 ℃ and the detection wavelength at 275 nm .Results The linear range of bergenin was in the range of 0 .0648-0 .648 μg (r=0 .9998) .The average recovery was 100 .24％ (RSD=1 .9％ ,n=6) .Conclusion The method is simple ,spe-cific and sensitive .It can be used to control the quality of Kangkeling mixture .