Stndy on diabetic rat therapy by islet transplantation with rat mesenchymal stem cells and scaffold of pig small intestinal submucosal / 中华器官移植杂志

ABSTRACT

Objective Engineer a scaffold with mesenchymal stem cells and small intestinal submucosal to evaluate the effect of islet transplantation in diabetic rats .Methods MSC and pancreatic islets were isolated from Sprague-Dawley rats ,and SIS was isolated from Bamei pigs .MSC were seeded on the SIS to construct MSC-SIS scaffold .The STZ-induced diabetic rats were divided into three groupsislets ,SIS ,and MSC-SIS .The expressions of insulin and CD31 were detected by immunofluorescence on the 14th day after transplantation ,and serum cytokines were detected by protein microarray .One-way ANOVA was used to compare the transplantation effect of each group .Results In MSC-SIS group ,the expressions of insulin and CD31 were significantly higher than those in the other two groups .Cytokines of VEGFA were increased while TNF-α,IFN-γand IL-6 decreased ,showing a significant difference (P<0 .05) .These results suggest that MSC-SIS scaffold significantly improve graft function and promote the expression of insulin and CD 31 , which may be related to the angiogenesis and anti-inflammatory effects of MSC .Conclusions Mesenchymal stem cells combined with intestinal submucosal scaffold can improve the effect of islet transplantation and provide a new method for the treatment of diabetes .