Effect of Modified Xiaoyaosan on ERK Signal Transduction Pathway of Dopamine Receptorin Model Rats with Hyperprolactinemia / 中国实验方剂学杂志

ABSTRACT

Objective:To explore the mechanism of modified Xiaoyaosan on extracellular regulated protein kinase(ERK) signal transduction pathway of central dopamine receptor in hyperprolactinemia(HPRL)model rats by treating them with Shugan Jianpi as the core. Method:The 96 SD female rats were randomly divided into normal group, model group, Bromocriptine group (0.001 g·kg-1), modified Xiaoyaosan high, medium and low dose group(60,30,15 g·kg-1), dopamine D1 receptor(D1R) antagonist group (SCH23390, SCH23390+ modified Xiaoyaosan group, SCH23390+bromocriptine group), dopamine D2 receptor(D2R) antagonist group (haloperidol group, haloperidol+modified Xiaoyaosan group,haloperidol+bromocriptine group).HPRL rat model was established by pituitary transplanta-tion. After 30 days of administration, renin-angiotensinsystem Ras and Raf protein expressions in thypothalamus of rats were detected by Western blot, and mitogen-activated protein kinase (MAPK) kinase 1/2(MEK1/2)mRNA and extracellular signaling regulates kinase 1/2(ERK1/2) mRNA expressions in the hypothalamus of rats were detected by Real-time PCR.The microstructures of mitochondria in ovarian granulosa cells were observed by electron microscopy. Result:The Ras,Raf protein,MEK1/2 and ERK1/2 mRNA expression were significantly decreased in model group compared with normal group (PPPPConclusion:Modified Xiaoyaosan stimulates D2R, inhibits D1R, regulates ERK/MAPK signaling pathway.It also effectively improve the mitochondrial function of ovarian granulosa cells and promote follicular development.It is suggested that the modified Xiaoyaosan with a multi-target mechanism in treating HPRL.