Induction of IL-8 by Chlamydia trachomatis through MAPK pathway rather than NF-kappaB pathway / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 307-313, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814449
ABSTRACT
OBJECTIVE@#To determine the signaling pathway required for Chlamydial induction of IL-8 expression in epithelial cells.@*METHODS@#The production and localization of IL-8 in Chlamydia-infected Hela 229 cells were monitored using Western blot, immunoflourescence, and ELISA. Activation of MAPK and NF-kappaB signaling pathways were detected by Western blot and immunoflourescence. The effect of different signaling pathways on Chlamydia-induced Il-8 was measured by experiments of chemical inhibitors.@*RESULTS@#IL-8 was induced by Chlamydia and was time-dependant. Chlamydial infection activated MAPK/ERK and MAPK/p38 pathways but not NF-kappaB pathway. Chlamydial induction of IL-8 was blocked by small molecule inhibitors targeting the ERK and p38 pathways.@*CONCLUSION@#Chlamydia-induced IL-8 in cervical epithelial cells, the natural target cell type of Chlamydia trachomatis infection, is dependent on MAPK pathway but not NF-kappaB pathway, which provides important information for further understanding the molecular mechanism of Chlamydia-induced inflammatory pathologies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
HeLa Cells
/
Signal Transduction
/
Chlamydia Infections
/
Chlamydia trachomatis
/
Interleukin-8
/
NF-kappa B
/
P38 Mitogen-Activated Protein Kinases
/
Epithelial Cells
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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