Proteomics analysis of adenosine A1 receptor agonist-induced delayed myocardial protection in rabbits / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 999-1002, 2011.
Article
in Zh
| WPRIM
| ID: wpr-814476
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the changes of myocardial protein expression profiles in 2-chloro-N6-cyclopentyladenosine (CCPA), an adenosine A1 receptor agonist-induced delayed myocardial protection in New Zealand rabbits .@*METHODS@#A total of 8 rabbits were randomly divided into a CCPA group (CCPA group) and a normal saline group (NS group). CCPA and NS were infused into rabbits in the CCPA group and the NS group respectively. Twenty-four hours later, the rabbits were subjected to 30 min left anterior descending coronary artery occlusion and were reperfused for 2 hours, then the ischemic zone tissues of left ventricle were sampled for proteomic analysis.A total of 12 other New Zeland rabbits were divided into a sham group (Sham group), a normal saline group (NS group) and a CCPA group (CCPA group). The expression of αB-crystalline, one of the differential proteins, was confirmed by Western blot.@*RESULTS@#Analysis of two dimensional gel electrophoresis showed that the expression of 55 protein spots were different between the two groups, 17 protein spots were preliminarily identified with the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Mascot and Expasy bioinformatics software. These proteins included stress proteins, metabolism-associated proteins, signal transduction pathway-related proteins, ionophorous proteins, immunity-associated proteins, and so on. Western blot showed that the expression of αB-crystalline was significantly up-regulated in the CCPA group.@*CONCLUSION@#The myocardial protein expression profiles are changed markedly in the preconditioning late phase of CCPA .The differential proteins might be involved in the delayed cardioprotection induced by CCPA.
Full text:
1
Index:
WPRIM
Main subject:
Myocardial Reperfusion Injury
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Adenosine
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Proteome
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Proteomics
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Therapeutic Uses
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Adenosine A1 Receptor Agonists
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Ischemic Postconditioning
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Metabolism
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Methods
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Myocardium
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2011
Type:
Article