Effect of bone morphogenetic protein-4 on the proliferation and differentiation of rat hepatic precursor cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 539-545, 2011.
Article
in Zh
| WPRIM
| ID: wpr-814552
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To determine the regulation effect of bone morphogenetic protein-4 (BMP-4) on the proliferation and differentiation of rat hepatic precursor cells.@*METHODS@#We used Noggin (200 ng/mL) as the function blocking control of BMP-4, and the hepatic precursor cells of WB-F344 were treated with recombinant BMP-4 at 50 ng/mL at different time points. The proliferation of WB-F344 cells were tested by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The ultrastructural characters of differentiated WB-F344 cells regulated by BMP-4 were observed under a transmission electron microscope. RT-PCR was used to examine mRNA expression of specific molecular markers for different cellular phenotypes potentially differentiated from the WB-F344 cells.@*RESULTS@#At different time points, the absorbance values in the BMP-4 treatment groups were higher than those in the control groups of Noggin and blank treatment (P<0.01). The WB-F344 cells treated with BMP-4 exhibited typical ultrastructural characters of well-differentiated epithelial cells. The hepatocyte mRNA markers were more significantly promoted in the differentiated WB-F344 cells in the BMP-4 treatment group than those in the other 2 control groups.@*CONCLUSION@#BMP-4 can promote the proliferation and directional differentiation towards hepatocytes of rat hepatic precursor cells of WB-F344.
Full text:
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Index:
WPRIM
Main subject:
Pharmacology
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Physiology
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Stem Cells
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Recombinant Proteins
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Carrier Proteins
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Cell Differentiation
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Cell Line
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Hepatocytes
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Cell Biology
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Cell Proliferation
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2011
Type:
Article