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Dynamic expression of E2F1 in lung of premature rats with hyperoxia-induced chronic lung disease and its significance / 中南大学学报(医学版)
Zhongnan Daxue xuebao. Yixue ban ; (12): 1008-1012, 2012.
Article in Zh | WPRIM | ID: wpr-814749
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To determine the dynamic expression of E2F1 in lung of premature rats with hyperoxia-induced chronic lung disease and the relation between E2F1 and pulmonary fibrosis.@*METHODS@#Premature Wistar rats at 21 days gestation were randomly and equally divided into a hyperoxia group and a room air group. The hyperoxia group was continuously exposed to hyperoxia (90%) while the air group in room air. Lung tissues in the 2 groups were obtained at 3, 7 and 14 days after exposing to either room air or hyperoxia. The changes of pulmonary histopathology at different time points were observed by hematoxylin and eosin staining; the severity of pulmonary fibrosis was evaluated; and the expression of E2F1 in lung tissue was detected by immunohistochemistry and Western blot.@*RESULTS@#After 3 days of hyperoxia, no significant interstitial fibrosis was observed; while after 7 days in the hyperoxia group, interstitial fibrosis was observed. These changes became more obvious after 14 days of prolonged hyperoxia exposure. No significant difference in the expressions of E2F1 protein was found between the hyperoxia group and the room air group 3 days postnatally (P>0.05). The expression of E2F1 in the hyperoxia group significantly increased 7 days and 14 days postnatally (P<0.05, P<0.01).@*CONCLUSION@#Abnormality of E2F1 expression is involved in the pathological process of the proliferation of lung fibroblasts in hyperoxia-induced chronic lung disease neonatal rats, and it plays an important role in lung fibrosis.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Pathology / Pulmonary Fibrosis / Immunohistochemistry / Rats, Wistar / Rats, Sprague-Dawley / Hyperoxia / E2F1 Transcription Factor / Lung / Lung Diseases / Animals, Newborn Limits: Animals Language: Zh Journal: Zhongnan Daxue xuebao. Yixue ban Year: 2012 Type: Article
Full text: 1 Index: WPRIM Main subject: Pathology / Pulmonary Fibrosis / Immunohistochemistry / Rats, Wistar / Rats, Sprague-Dawley / Hyperoxia / E2F1 Transcription Factor / Lung / Lung Diseases / Animals, Newborn Limits: Animals Language: Zh Journal: Zhongnan Daxue xuebao. Yixue ban Year: 2012 Type: Article