Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 144-149, 2015.
Article
in Zh
| WPRIM
| ID: wpr-815199
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.@*METHODS@#A total of 60 SD rats were randomly divided into 8 groups A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.@*RESULTS@#Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).@*CONCLUSION@#PULC may inhibit the FLS proliferation in AA rats by increase in FLS apoptosis.
Full text:
1
Index:
WPRIM
Main subject:
Arthritis, Experimental
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Synovial Membrane
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Chemistry
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Interleukin-8
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Interleukin-6
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Rats, Sprague-Dawley
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Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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Cell Biology
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Pulsatilla
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2015
Type:
Article