Tauroursodeoxycholic acid suppresses endoplasmic reticulum stress in pulmonary tissues of intermittent hypoxia mice / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1165-1172, 2015.
Article
in Zh
| WPRIM
| ID: wpr-815359
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the mechanism of tauroursodeoxycholic acid (TUDCA) in suppressing apoptosis in pulmonary tissues of intermittent hypoxia (IH) mice model.
@*METHODS@#A total of 32 C57 mice were randomly divided into a control group, a TUDCA group, an IH group and an IH+TUDCA group (8 mice per group). The mice were put in specially designed chambers and exposed to IH treatment for 4 weeks. In the chambers, oxygen levels repeatedly decreased from 21% to 10% and recovered from 10% to 21%, lasting for 8 hours in every day. After 4 weeks of IH exposure, the expression levels of caspase-12 and cleaved caspase-3 in pulmonary tissues were detected by Western blot. Meanwhile, the expression levels of glucose regulated protein-78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP) were quantified by Western blot, immunochemistry and real-time PCR.
@*RESULTS@#Compared with the control group, the expression levels of caspase-12, cleaved caspase-3, GRP78 and CHOP were increased in the IH group (all P<0.01). TUDCA treatment could reduce these proteins expression (all P<0.05).
@*CONCLUSION@#Endoplasmic reticulum stress-mediated apoptosis can be activated in pulmonary tissues after chronic IH exposure, and TUDCA can reduce the cellular apoptosis via suppressing endoplasmic reticulum stress.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Taurochenodeoxycholic Acid
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Apoptosis
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Disease Models, Animal
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Transcription Factor CHOP
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Caspase 3
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Caspase 12
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Real-Time Polymerase Chain Reaction
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Endoplasmic Reticulum Stress
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Heat-Shock Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2015
Type:
Article