Simultaneous Determination of 6 Kinds of Constituent in Dahuang Zhechong Pills by HPLC-PDA / 中国药房

ABSTRACT

OBJECTIVE： To establish a method for amygdalin， paeoniforin， baicalin， glycyrrhizic acid， emodin and chrysophanol in Dahuang zhechong pills. METHODS： HPLC method was adopted. The determination was performed on Welchrom C18 with mobile phase consisted of methanol-0.1％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃， and the detection wavelengths were set at 210 nm （amygdalin）， 230 nm （paeoniforin， baicalin） and 250 nm [glycyrrhizic acid （calculated with ammonium glycyrrhizinate）， emodin， chrysophanol]. The sample size was 10 μL. RESULTS： The linear ranges of amygdalin，paeonifiorin，baicalin，ammonium glycyrrhizinate， emodin and chrysophanol fell within the ranges of 21.028-157.71，2.052-90.390， 34.288-257.16， 8.252 0-61.890， 3.272 0-24.540，4.768 0-35.760 μg/mL （all r≥0.999 2）. The limits of detection were 0.105 0， 0.121 0， 0.068 6， 0.082 5， 0.024 6， 0.017 9 μg/mL. The limits of quantitation were     0.263 0， 0.362 0， 0.171 0， 0.268 0， 0.065 5， 0.047 7 μg/mL. RSDs of precision， stability（12 h）and reproducibility tests were lower than 2.00％ （n＝6）. The  average  recoveries were 96.01％-100.76％ （RSD＝1.63％，n＝6）， 97.09％-101.86％ （RSD＝1.50％，n＝6）， 99.70％-101.99％（RSD＝0.82％，n＝6），96.29％-99.52％（RSD＝1.35％，n＝6）， 98.47％-102.14％ （RSD＝1.35％， n＝6） and 97.19％-99.16％（RSD＝0.69％，n＝6），respectively. CONCLUSIONS： The method is accurate， simple and reproducible for simultaneous determination of amygdalin， paeoniforin， baicalin， glycyrrhizic acid， emodin and chrysophanol in Dahuang zhechong pills.