Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid
Asian Pacific Journal of Tropical Medicine
; (12): 473-480, 2013.
Article
in En
| WPRIM
| ID: wpr-820018
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To identify target genes of transcription factor CCAAT enhancer-binding protein β (CEBPB) in acute promyelocytic leukemia cells induced by all-trans retinoic acid.@*METHODS@#A new strategy for high-throughput identification of direct target genes was established by combining chromatin immunoprecipitation (ChIP) with in vitro selection. Then, 106 potential CEBPB binding fragments from the genome of the all-trans retinoic acid (ATRA)-treated NB4 cells were identified.@*RESULTS@#Of them, 82 were mapped in proximity to known or previously predicted genes; 7 were randomly picked up for further confirmation by ChIP-PCR and 3 genes (GALM, ITPR2 and ORM2) were found to be specifically up-regulated in the ATRA-treated NB4 cells, indicating that they might be the down-stream target genes of ATRA.@*CONCLUSIONS@#Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Tretinoin
/
Binding Sites
/
Leukemia, Promyelocytic, Acute
/
Signal Transduction
/
Gene Expression Regulation, Neoplastic
/
Cell Differentiation
/
Cell Line
/
Polymerase Chain Reaction
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2013
Type:
Article