Molecular epidemiology and characterization of Streptococcus mutans strains in Korea / 대한구강보건학회지
Journal of Korean Academy of Oral Health
;
: 34-40, 2020.
Article
in English
| WPRIM
| ID: wpr-820819
ABSTRACT
OBJECTIVES:
We investigated the characteristics of Streptococcus mutans in the national culture collection from Korea. Twenty-nine (dental plaque, n=27; endodontic infections, n=1; blood, n=1) isolates were included in this study.METHODS:
Antimicrobial susceptibilities were tested using the disk diffusion test. Multilocus sequence typing (MLST), serotyping, and collagen-binding genes were used for polymerase chain reaction (PCR) and direct sequencing. A collagen-binding (to assess the adhesion properties) assay was performed. S. mutans demonstrated high susceptibility to antimicrobial agents. Differences in collagen-binding abilities of the cnm-positive and -negative groups were compared using the Mann-Whitney U test (P<0.05).RESULTS:
MLST analyses revealed 25 sequence types (STs), 17 of which (ST213-ST229) contained new alleles. The strains were classified into four serotypes with the c type encompassing 79.3% of all strains, while the e, f, and k types representing 6.9% each. Analysis of the cnm and cbm genes, which encode the two surface adhesin components of S. mutans, revealed three cnm-positive strains, each displaying greater adhesion ability than those of the cnm-negative strains.CONCLUSIONS:
This study highlights the presence of a wide variety of S. mutans genotypes in Korea. These findings may provide useful information regarding the pathogenesis of infectious diseases, such as dental caries.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Streptococcus
/
Streptococcus mutans
/
Serotyping
/
Polymerase Chain Reaction
/
Communicable Diseases
/
Bacteremia
/
Molecular Epidemiology
/
Dental Caries
/
Diffusion
/
Alleles
Type of study:
Screening study
Country/Region as subject:
Asia
Language:
English
Journal:
Journal of Korean Academy of Oral Health
Year:
2020
Type:
Article
Similar
MEDLINE
...
LILACS
LIS