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Analysis of carnosic acid metabolites in rats by UHPLC-Q-Exactive MS / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3952-3960, 2020.
Article in Chinese | WPRIM | ID: wpr-828362
ABSTRACT
A method of ultra-high performance liquid chromatography coupled with quadrupole/electrostatic field Obitrap high-resolution mass spectrometry(UHPLC-Q-Exactive MS) was established to comprehensively identify the metabolites of carnosic acid in rats. After oral gavage of carnosic acid CMC-Na suspension in rats, urine, plasma and feces samples were collected and pretreated by solid phase extraction(SPE). Acquity UPLC BEH C_(18 )column(2.1 mm×100 mm, 1.7 μm) was used with 0.1% formic acid solution(A)-acetonitrile(B) as the mobile phase for the gradient elution. Biological samples were analyzed by quadrupole/electrostatic field Obitrap high-resolution mass spectrometry in positive and negative ion mode. Based on the accurate molecular mass, fragment ion information, and related literature reports, a total of 28 compounds(including carnosic acid) were finally identified in rat samples. As a result, the main metabolic pathways of carnosic acid in rats are oxidation, hydroxylation, methylation, glucuronide conjugation, sulfate conjugation, S-cysteine conjugation, glutathione conjugation, demethylation, decarbonylation and their composite reactions. The study showed that the metabolism of carnosic acid in rats could be efficiently and comprehensively clarified by using UHPLC-Q-Exactive MS, providing a reference for clarifying the material basis and metabolic mechanism of carnosic acid.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mass Spectrometry / Chromatography, High Pressure Liquid / Abietanes / Solid Phase Extraction Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mass Spectrometry / Chromatography, High Pressure Liquid / Abietanes / Solid Phase Extraction Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article