A human circulating immune cell landscape in aging and COVID-19
Protein & Cell
; (12): 740-770, 2020.
Article
in En
| WPRIM
| ID: wpr-828746
Responsible library:
WPRO
ABSTRACT
Age-associated changes in immune cells have been linked to an increased risk for infection. However, a global and detailed characterization of the changes that human circulating immune cells undergo with age is lacking. Here, we combined scRNA-seq, mass cytometry and scATAC-seq to compare immune cell types in peripheral blood collected from young and old subjects and patients with COVID-19. We found that the immune cell landscape was reprogrammed with age and was characterized by T cell polarization from naive and memory cells to effector, cytotoxic, exhausted and regulatory cells, along with increased late natural killer cells, age-associated B cells, inflammatory monocytes and age-associated dendritic cells. In addition, the expression of genes, which were implicated in coronavirus susceptibility, was upregulated in a cell subtype-specific manner with age. Notably, COVID-19 promoted age-induced immune cell polarization and gene expression related to inflammation and cellular senescence. Therefore, these findings suggest that a dysregulated immune system and increased gene expression associated with SARS-CoV-2 susceptibility may at least partially account for COVID-19 vulnerability in the elderly.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pneumonia, Viral
/
Mass Spectrometry
/
Aging
/
Gene Rearrangement
/
CD4-Positive T-Lymphocytes
/
Cytokines
/
Sequence Analysis, RNA
/
Coronavirus Infections
/
Gene Expression Regulation, Developmental
/
Cell Lineage
Type of study:
Prognostic_studies
Limits:
Adult
/
Aged
/
Aged80
/
Humans
Language:
En
Journal:
Protein & Cell
Year:
2020
Type:
Article