Down-regulated Reactive Oxygen Species by Heat Shock Protein 90 in 3-Hydroxykynurenine-induced SKN-SH Cell Death / 체질인류학회지
Korean Journal of Physical Anthropology
; : 231-240, 2004.
Article
in Ko
| WPRIM
| ID: wpr-83423
Responsible library:
WPRO
ABSTRACT
In this present study, we show that 3HK-induced reactive oxygen species (ROS) accumulation and caspase activation lead to apoptotic cell death. Pretreatment with N-acetylcysteine (NAC), an effective antioxidant, significantly attenuated 3HK-induced apoptosis by way of a reduction of ROS accumulation and caspase activity. SKN-SN cells were protected from 3HK-induced cytotoxicity by heat shock protein (HSP). HSP90 effectively attenuated 3HK-mediated ROS accumulation and apoptosis. In addition, the protective effect of HSP90 was abolished by pretreatment with HSP90 anti-sense oligonucleotide, but not when pretreated with anti-senses for other HSPs. These results suggest that HSP90 protects SKN-SH cells from 3HK-induced cytotoxicity by reducing ROS levels and caspase activity.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Acetylcysteine
/
Cell Death
/
Reactive Oxygen Species
/
Apoptosis
/
Hot Temperature
/
Heat-Shock Proteins
Language:
Ko
Journal:
Korean Journal of Physical Anthropology
Year:
2004
Type:
Article