Ubiquitin ligase TRIM46 promotes the proliferation and invasion of colorectal cancer cells / 肿瘤
Tumor
;
(12): 334-339, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-848562
ABSTRACT
Objective:
To explore the effect of tripartite motif 46 (TRIM46) on the proliferation and invasion of colorectal cancer cells and study its possible mechanism.Methods:
After TRIM46 siRNA was transfected into human colorectal cancer HCT116 cells, the expressions of TRIM46 mRNA and protein were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. CCK-8 assay and Transwell chamber assay were implemented to observe the effect of TRIM46 down-regulation on the proliferation and invasion of HCT116 cells, and the expression of Src homology 2 (SH2)-containing tyrosine phosphatase 1 (SHP1) was detected by Western blotting.Results:
After TRIM46 siRNA was transfected into human colorectal cancer HCT116 cells, the expression levels of TRIM46 mRNA and protein were inhibited (both P < 0.05). Knockdown of TRIM 46 significantly suppressed the cell proliferation and invasion of HCT116 cells (both P < 0.05). However, the expression level of SHP1 was increased (P < 0.05).Conclusion:
Ubiquitin ligase TRIM46 may improve the proliferation and invasion of human colorectal cancer cells through suppressing SHP1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2017
Type:
Article
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