Therapeutic effects and spectra-effect relationship of different extracts of Eucommia ulmoides on Parkinson’s disease mice / 中草药

ABSTRACT

Objective: To explore the therapeutic effects of different extracts of Eucommia ulmoides on Parkinson’s disease mice, as well as the relationship between ultra-high performance liquid chromatography (UPLC) fingerprint and treatment of Parkinson’s disease. Methods Through the mouse climbing test and the content of dopamine (DA) in the striatum of the brain, the therapeutic effect of different gradient ethanol extracts of E. ulmoides on Parkinson’s disease mice was observed. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to analyze the fingerprints of different extracts of E. ulmoides. Combined with the results of climbing rod test and dopamine content, partial least squares regression (PLSR) analysis was used to establish the pharmacodynamic relationship between E. ulmoides and Parkinson’s disease. Results The 50% and 75% ethanol extracts of E. ulmoides could significantly shorten the climbing time. The 75% ethanol extract of E. ulmoides significantly increased the striatum dopamine content in the brain. The results of PLSR analysis showed that ulmoside, liriodendrin, 5-hydroxymethylfurfural, caffeic acid in E. ulmoides were closely related to climbing rod and dopamine content of mice. Conclusion The ethanol extract of E. ulmoides has anti-Parkinson’s disease effect, and the effect is most significant with 75% alcohol extract. The compounds of ulmoside, liriodendrin, 5-hydroxymethylfurfural, caffeic acid may be the main active ingredients of E. ulmoides in the treatment of Parkinson’s disease.