Identification of miRNA target genes in Panax ginseng by degradome sequencing analysis / 中草药

ABSTRACT

Objective:To analyze and identify ginseng miRNA. Methods: The miRNA and target genes of Shizhu ginseng and Yuan ginseng were detected by the degradation sequencing technology; Functional annotation of Degradome genes was carried out using public databases of KEGG/NR/GO database; The expression of miRNA and target genes of Shizhu ginseng and Yuan ginseng was determinated by real time fluorescence quantitative PCR technique. Results: A total of 13 target genes of eight miRNA families were obtained; The target gene type of miRNA was mainly transcriptional factor, response factor, and signal transduction pathway by means of KEGG/NR/GO database analysis. The results of real time fluorescence quantitative PCR verification of aqc-miR-159, bdi-miR162, cpa-miR319, pgi-miR4376, smo-miR396 and its target genes were basically consistent with the expression of miRNA and target genes from the degradation group. Conclusion: The target genes of partial Panax ginseng miRNA is clarified, which lays the foundation for further study of the possible function of ginseng miRNA.