Urine metabonomic study on Danzhi Jiangtang Capsule in treatment of type 2 diabetic rats based on UPLC/QTOF-MS / 中草药

ABSTRACT

Objective: To observe the metabolic profile changes caused by Danzhi Jiangtang Capsule (DJC) in treatment of type 2 diabetic rats and to study the possible metabolism. Methods: All the SD rats involved in the experiment were randomly divided into normal control, model, and DJC groups. The rats in the model and DJC groups were fed on high-fat diet for 4 weeks after ip injection with a small dose of streptozotocin to copy type 2 diabetic rat model. After the modeling, the rats in the DJC group were ig administered with DJC (1.26 g/kg) for 4 weeks, and the blood glucose was monitored weekly. After the treatment, urine of rats was collected, then all the rats were sacrificed, abdominal aortic blood was taken to detect the levels of insulin (INS) and glycated hemoglobin (GHb) in plasma. Using UPLC/QTOF-MS with principal component analysis (PCA) to analyze the metabolic profile changes, the metabolites were identified by databases, and metabolism pathways were analyzed. Results: Blood glucose monitoring and INS, GHb testing results showed that DJC could lower blood sugar and elevate plasma INS level. In the positive and negative ion modes, we screened seven and six variability markers respectively. The variability markers were related to glucose metabolism, tyrosine metabolism tryptophan metabolism, and oxidative stress-related reactions. Conclusion: DJC can lower blood sugar, repair islet cells to cure the type 2 diabetes, and the possible mechanism may relate to the regulation of energy metabolism, tyrosine metabolism, tryptophan metabolism, and oxidative stress.