Spectrum-effect relationship between fingerprint in plasma of Heishunpian and its anti-heart failure effect / 中草药

ABSTRACT

To investigate the spectrum-effect relationship between the fingerprint in plasma of Heishunpian (black-processed pieces of Aconit Lateralis Radix Praeparata) and its pharmacodynamic parameters (blood pressure and heart rate) after ig administration to rats, and to elucidate its effective substances. Ultra performance liquid chromatography with quadrupole and time-of-flight mass spectrometry (UPLC-QTOF/MS) was used for fingerprint profiling by analyzing plasma samples obtained after ig administration of the ethanol extract from Heishunpian to rats. Meanwhile, the blood pressure and heart rate of heart-failure rats were continuously monitored and recorded. The relationship between the absorbed fingerprint and pharmacodynamic parameters of Heishunpian was established by correlation analysis with partial least square regression (PLSR) method. A total of 13 alkaloids were detected in rat plasma, among which hypaconine, benzoylmesaconitine, benzoylhypaconitine, and 3, 13-deoxybenzoylaconitine were found to be significantly and positively related to the pharmacodynamic data with VIP > 1. Hypaconine, benzoylmesaconitine, benzoylhypaconitine, and 3, 13-deoxybenzoylaconitine are potential effective substances with anti-heart failure effects of Heishunpian.