Chemical constituents of Sabia fasciculata / 中草药

ABSTRACT

Objective: To investigate the chemical constituents in the dried leaves of Sabia fasciculata. Methods: Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR and 13C-NMR, and physical constants were used to elucidate their structures. Results: Fifteen compounds were isolated from 95% ethanol extracts of S. fasciculata, including seven pentacyclic triterpenoids, such as methyl-3-oxo-olean-12-ene-28-oate (1), betulin (2), 3-oxo-olean-Δ11,13(18)-diene (3), oleanolic acid (4), imberic acid (5), pseudo- ginsenoside RP1 (6), and chikusetsusaponin IVa (7); three flavonoids, such as quercetin (8), rutin (9), and mutabiloside (10); three alkaloids, such as fuseine (11), N-p-feruloyltyramine (12), and N-trans-coumaroyl tyramine (13); two steroids, such as β-sitosterol (14) and β-daucosterol (15). Conclusion: All the compounds are isolated from the plant for the first time. Compounds 1, 5-7, and 10 are isolated from the plants of Sabia Colelbr. for the first time.