HPLC Fingerprint of Fructus Aristolochiae and Honey Processed Products from Different Habitats / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1397-1402, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-858605
ABSTRACT
OBJECTIVE:
To establish the HPLC fingerprints of Fructus Aristolochiae and honey-fried products from different habitats, compare and analyze their difference in chemical components.METHODS:
Using an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 μm), HPLC method was performed to collect the fingerprints. The mobile phase A and B were acetonitrile and 1% acetic acid, respectively. Gradient elution was performed at the flow rate of 1.0 mL·min-1 and column temperature of 30℃. The detection was carried out at 250 nm. Similarity Evaluation System for Chromatographic Fingerprint of TCMs (Ver. 2012) and SPSS22.0 was used in cluster analysis and principal component analysis (PCA).RESULTS:
The fingerprints of 14 batches of Fructus Aristolochiae samples, before and after process, were established by HPLC. The reduction rate of AA I, a poisonous component, was over 36%. Except for S4 (0.822), S18 (0.771) and S19 (0.639), the similarities of Fructus Aristolochiae and processed products were over 0.9. The non-processing samples were classified into four groups by cluster analysis. As PCA shown, the five principal components including AA I had a contributing rate of 84.914% to cumulative sum of squares, illustrating the chemical similarity and difference of samples from different areas.CONCLUSION:
The results may provide reference for Fructus Aristolochiae processing and standardization of its detoxification technology.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2017
Type:
Article
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